| 质粒名称: | pH2B-mScarlet3-H (aka mYongHong) |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000752 |
| 产品用途: | Expresses H2B-mScarlet3-H in mammalian cells |
| 存储实验室: | Zhifei Fu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pN1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | H2B, mScarlet3-H |
| 物种来源: | H.sapiens, Synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A highly stable monomeric red fluorescent protein for advanced microscopy
Xiong H., Chang Q., Ding J., Wang S., Zhang W., Li Y., Wu Y., Lin P., Yang C., Liu M., Fang G., Yang Y., Xie J., Qi D., Jiang T., Fu W., Hu F., Chen Y., Yue R., Li Y., Cui Y., Li M., Fan S., Yang Y., Xu Y., Li D., Zhang F., Zhao H., Wu C., Zheng Q., Piatkevich KD., Fu Z.
Nat. Methods 2025 1–11 (2025)
|
pH2B-mScarlet3-H (aka mYongHong)
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pH2B-mScarlet3-H (aka mYongHong)载体质粒基本信息
pH2B-mScarlet3-H (aka mYongHong)质粒图谱载体图谱和pH2B-mScarlet3-H (aka mYongHong)载体序列质粒序列多克隆位点信息
载体图谱.png)
pH2B-mScarlet3-H (aka mYongHong)质粒载体简介
pH2B-mScarlet3-H (aka mYongHong)质粒序列载体序列
LOCUS pH2B-mScarlet3-H (aka mYongHong) 5014 bp DNA circular SYN 30-DEC-2024
DEFINITION TSN20241013-0571-00010_752_20241014-BAN4_C03_C03_i20.1.
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5014)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5014
/mol_type="other DNA"
/organism="synthetic DNA construct"
rep_origin 214..802
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
enhancer 977..1280
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1281..1484
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 1513..1890
/codon_start=1
/label=H2B
/translation="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKV
LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
GELAKHAVSEGTKAVTKYTSSK"
regulatory 1900..1909
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 1906..2592
/codon_start=1
/label=mScarlet3-H
/translation="MDSTEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
LRVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYWKQSFPEGFKWERVMNFEDGGA
VSVAQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDVVLKGDIKH
ALRLKDGGRYLADFKTTYRAKKPVQMPGAFNIDRKLDITSHNEDYTVVEQYERSVARHS
TGGSGGS"
polyA_signal 2718..2839
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(2846..3301)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3328..3432
/gene="bla"
/label=AmpR promoter
promoter 3434..3791
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3642..3777
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3826..4620
/codon_start=1
/product="aminoglycoside phosphotransferase"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 4852..4899
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
1 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
61 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
121 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
181 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
241 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
301 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
361 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
421 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
481 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
541 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
601 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
661 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
721 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
781 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
841 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
901 tattaccgcc atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc
961 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
1021 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
1081 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
1141 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
1201 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
1261 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
1321 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
1381 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
1441 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc
1501 agatccgcta gcatgcctga accggcaaaa tccgctccgg cccctaaaaa gggctccaag
1561 aaagccgtca ccaaagccca gaagaaagac ggcaagaagc gcaagcgcag ccgcaaagag
1621 agctactcca tctacgtgta caaggtgctg aagcaggtcc accccgacac cggcatctcg
1681 tccaaggcca tgggcatcat gaactccttc gtcaacgaca tcttcgagcg catcgcggga
1741 gaggcttccc gcctggcgca ctacaacaag cgctccacca tcacatcccg cgagatccag
1801 acggccgtgc gcctgctgct gcccggcgag ctggccaagc acgccgtgtc cgagggcacc
1861 aaggcggtca ccaagtacac cagctccaag ccaccggtcg ccaccatgga tagcaccgag
1921 gcagtgatca aggagttcat gcggttcaag gtgcacatgg agggctccat gaacggccac
1981 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
2041 ctgagggtga ccaagggtgg ccccctgccc ttctcctggg acatcctgtc ccctcagttc
2101 atgtacggct ccagggcctt cacgaagcac cccgccgaca tccccgacta ctggaagcag
2161 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgccgtg
2221 tccgtggccc aggacacctc cctggaggac ggcaccctga tctacaaggt gaagctccgc
2281 ggcaccaact tccctcctga cggccccgta atgcagaaga agacaatggg ctgggaagca
2341 tccaccgagc ggttgtaccc cgaggacgtc gtgctgaagg gcgacattaa gcatgccctg
2401 cgcctgaagg acggcggccg gtacctggcg gacttcaaga ccacctacag ggccaagaag
2461 cccgtgcaga tgcccggcgc cttcaacatc gaccgcaagt tggacatcac atcccacaac
2521 gaggactaca ccgtggtgga acagtacgaa cgctccgtgg cccgccactc caccggcggc
2581 tccggtggct cctaaagcgg ccgcgactct agatcataat cagccatacc acatttgtag
2641 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
2701 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
2761 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
2821 aactcatcaa tgtatcttaa ggcgtaaatt gtaagcgtta atattttgtt aaaattcgcg
2881 ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
2941 tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
3001 ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
3061 ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca
3121 ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
3181 gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
3241 gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
3301 tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
3361 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
3421 aaaaggaaga gtcctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
3481 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
3541 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
3601 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
3661 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
3721 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
3781 gcttttgcaa agatcgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat
3841 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca
3901 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg
3961 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcaaga cgaggcagcg
4021 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact
4081 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct
4141 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg
4201 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt
4261 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc
4321 gcgccagccg aactgttcgc caggctcaag gcgagcatgc ccgacggcga ggatctcgtc
4381 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga
4441 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc
4501 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt
4561 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga
4621 gcgggactct ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt
4681 tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg
4741 gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac cctaggggga
4801 ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa
4861 aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg gttcggtccc
4921 agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac gcccgcgttt
4981 cttccttttc cccaccccac cccccaagtt cggg
//
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